Anti-Hu CD95 Purified FAS Monoclonal Antibody

Product Name

Anti-Hu CD95 Purified FAS Monoclonal Antibody

View all FAS Antibodies

SKU/Catalog Number

M00055-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD95 Purified FAS Monoclonal Antibody (Catalog# M00055-1). Tested in Flow Cytometry, IHC-P application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD95 Purified FAS Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00055-1)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

LT95

Isotype

Mouse IgG1

Immunogen

HUT-78 human T cell lymphoma cell line. The antibody LT95 reacts with an extracellular epitope on CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00055-1 is reactive to FAS in Human

Applications

M00055-1 is guaranteed for Flow Cytometry, IHC-P Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

37.732kDa

Background of FAS

CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-12 µg/ml. Excellent.
Immunohistochemistry (paraffin sections): Positive tissue: tonsil.

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry validation of FAS using Anti-Hu CD95 Purified FAS Monoclonal Antibody (M00055-1).

Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE).
Histograms:
Red – Isotype mouse IgG1 control staining
Black – standard anti-CD95 monoclonal antibody (DX2)
Green – anti-CD95 (LT95)

A – JURKAT human peripheral blood T cell leukemia cell line
B – RAMOS human Burkitt lymphoma cell line
C – CEM human leukemia cell line
D – MOLT-4 human acute lymphoblastic T cell leukemia cell line

Gene/Protein Information For FAS (Source: Uniprot.org, NCBI)

Alternative Names

ALPS1A; APO-1 cell surface antigen; Apo-1; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; APT1; CD95 antigen; CD95; Fas (TNF receptor superfamily, member 6); Fas AMA; FAS1; FASLG receptor; FASTM; mutant tumor necrosis receptor superfamily member 6; TNF receptor superfamily member 6; TNFRSF6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6 FAS ALPS1A, APO-1, APT1, CD951, FASTM, TNFRSF6, FAS Fas cell surface death receptor tumor necrosis factor receptor superfamily member 6|APO-1 cell surface antigen|CD95 antigen|FASLG receptor|Fas (TNF receptor superfamily, member 6)|Fas AMA|TNF receptor superfamily member 6|apoptosis antigen 1|apoptosis signaling receptor FAS|apoptosis-mediating surface antigen FAS|mutant tumor necrosis receptor superfamily member 6|tumor necrosis factor receptor superfamily, member 6

For more info on FAS, check out the FAS Infographic

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In this infographic, you will see the following information for FAS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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